New antibiotics, carbazomycins A and B. III. Taxonomy and biosynthesis.

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Abstract
The carbazomycin-producing microorganism, strain H 1051-MY 10, was determined to be a strain of Streptoverticillium ehimense. Biosynthesis of carbazomycin B was studied using 14C-labeled and 13C-enriched precursors in combination with 13C NMR spectroscopy. The C-2 carbon of [2-13C]tryptophan was involved at the C-3 carbon in carbazomycin B; both carbons of [1,2-13C]acetate were involved at the C-1 and C-10 moiety of the antibiotic. [CH3-13C]methionine was involved at the methoxyl group, but not at the methyl group, on the C-2 carbon of the antibiotic. Neither [1-14C]tryptophan nor [2,3-13C]propionic acid was detected in the antibiotic; a progenitor of the C-2 and C-11 moiety of the antibiotic was not determined.