Localization of fibrillarin and nucleolin in nucleoli of mouse preimplantation embryos

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Abstract
The localization of fibrillarin and nucleolin in the nuclei of mouse two‐cell, four‐cell, and eight‐cell embryos has been studied using immunofluorescent staining with specific antibodies. In all of these cleavage stages, both antigens were associated exclusively with the peripheral region of the nucleolus precursor bodies (NPBs). The original speckled fluorescent staining pattern in the early two‐cell stage was progressively changed into a continuous fluorescent‐positive layer localized in the cortex of the NPBs in the four‐cell embryos. The compact central area of NPBs was never stained. Both proteins were colocalized in the same substructures of developing nucleoli. In order to analyze the interaction of chromatin, with NPBs, DNA structures were specifically immunolbelled. At the time of resumption of nucleolar transcription (in the two‐cell mouse embryo), DNA was detected at the periphery of, but not penetrating into, NPBs. Our results confirm the view that the cortical region of NPBs could represent a nucleolonemal area involved in the resumption of nucleolar transcription in the early mouse embryo.