The free and esterified sterol present in bovine adrenal cortex and medulla

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Abstract
Extracts (diethyl ether-ethanol of bovine adrenal cortex and medulla were made and fractionated on Florisil to give free and esterified sterol fractions. The minor sterols were concentrated by crystallization from boiling acetic acid and investigated by thin-layer and gas-liquid chromatography. The cholesterol ester fatty acids of the cortex and medulla were studied. The major fatty acids in the cortex are (values as percentages of the total):llnoleic acid, 21.7; oleic acid, 21.1; palmitic acid, 20. The same major acids appear in the medulla, their respective percentage weights being 28.2, 14.8 and 19. The cortex contains (mg/100 g fresh wt.): cholesterol, 286, of which 15.4% is esterified; cholestanol, 0. 230; methostenol, 0. 460, of which 55% is esterified; and trace amounts of other ''companion'' sterols. The medulla contains (mg/100 g. fresh wt,): cholesterol, 415, of which 6. 5% is esterified; cholestanol, 0. 231; lanosterol, 0. 392; and trace quantities of other sterol ''companions''.

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