STUDIES IN HISTOCHEMISTRY: QUANTITATIVE HISTOLOGICAL DISTRIBUTION OF CHOLESTEROL IN ADRENAL GLANDS OF THE COW, RAT AND MONKEY, AND EFFECTS OF STRESS CONDITIONS, ACTH, CORTISONE AND DESOXYCORTICOSTERONE1,2

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Abstract
AS PART of a broader program dealing with the quantitative histochemical basis for the functions of the adrenal gland, this study on cholesterol extends recent work on ascorbic acid (Bahn and Glick, 1954a, b), potassium and lipid (Glick et al., 1955), phenolsulfatase (Glick et al., 1954), enzymatic conjugation of m-aminophenol with sulfate and glucuronate (Glick and Stecklein, 1954), β-glucuronidase (Wattenberg and Glick, 1953; Nayyar and Glick, 1955), and coenzyme A (Malmström and Glick, 1954). The fall in the cholesterol content of the adrenal cortex, like that of ascorbic acid, has been taken as an indication of adrenal cortical stimulation and, as with ascorbic acid, the previous quantitative work on cholesterol dealt with analyses of whole glands or whole cortices (Long, 1947). The present investigation was designed to carry the analyses to the individual zones so that a histological differentiation of the quantitative effects could be made.