A Comparison of Benzo(A)Pyrene-4,5-Epoxide Hydrase Activity in Hamster Embryo Cells, Hepatocytes and Livers Using High-Pressure Liquid Chromatography

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Abstract
The benzo(a)pyrene-4,5-epoxide (BP-4,5-epoxide) hydrase activities of intact hamster hepatocytes and embryo cells, and homogenates of these cells and of adult liver are compared. The product of the epoxide hydrase (EH) reaction, trans-4,5-dihydro-4,5-dihydroxybenzo(a)pyrene (BP-4,5-diol) [a carcinogen], was isolated by high-pressure liquid chromatography (HPLC) with a Waters Bondapak C18/Corasil column and acetonitrile-water as the mobile phase. Using this procedure to determine BP-4,5-epoxide hydrase activity in intact cells, 266 nmol of BP-4,5-diol/106 cells were produced by hepatocytes while no diol formation was detected with embryo cells. EH activity in the intact hepatocytes was 8-fold greater than in hepatocyte homogenates.