Comparative Study of Bacterial Groups within the Human Cecal and Fecal Microbiota
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Open Access
- 1 October 2001
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 67 (10) , 4939-4942
- https://doi.org/10.1128/aem.67.10.4939-4942.2001
Abstract
The composition of the human cecal microbiota is poorly known because of sampling difficulties. Samples of cecal fluid from eight subjects were collected via an intestinal tube. Feces were also collected. Total anaerobes, facultative anaerobes, bifidobacteria, andBacteroides were enumerated by culture methods, and the predominant phylogenetic groups were quantified by molecular hybridization using a set of six rRNA-targeted probes. The numbers of strict anaerobes, bifidobacteria, Bacteroides, and members of the Clostridium coccoides group and Clostridium leptum subgroup were lower in the cecum. Facultative anaerobes represented 25% of total bacteria in the cecum versus 1% in the feces.Keywords
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