8006-I, an antibiotic from Amblyosporium spongiosum (Pers.) Hughes sensu Pirozynski. II. Biological properties.

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Abstract
8006-I is an antibacterial antibiotic with a rather broad spectrum of activity. The minimum inhibitory concentrations for the most sensitive bacteria are in the range of 1-10 .mu.g/ml. Yeasts are not affected by concentrations up to 100 .mu.g/ml. Some filamentous fungi like Fusarium oxysporum and Mucor miehei are inhibited at 100 .mu.g/ml. In [mouse] Ehrlich carcinoma ascitic cells, the incorporation of uridine and leucine and, to a lesser extent, that of thymidine is reduced. In isolated nuclei of these cells, the incorporation of UTP into RNA is inhibited. At low concentrations, the incorporation of uracil into trichloroacetic acid-precipitable material is almost completely inhibited in cells of Bacillus subtilis; at higher concentrations all macromolecular synthesis are affected. No reduction of respiration of the cells is observed. The antibiotic exhibits weak hemolytic activity and lytic activity towards bacteria. In vitro an inhibition of DNA- and RNA-polymerase from Escherichia coli is observed. Poly(U)-directed poly(Phe) synthesis is not affected.