Chlorophyll Synthesis in Chlorella II. Effect of Glucose and Light Intensity on the Lag Phase

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Abstract
The lag phase in chlorophyll synthesis which occurs upon dilution of a dense illuminated culture of Chlorella was related to the response of the cell to its new environment. Synthesis was stimulated during and after the lag phase if the cells were grown at lower than the usual light intensity. If the diluted culture contained glucose, the lag phase was lengthened. As glucose was depleted chlorophyll synthesis commenced. Glucose had no lasting effect on this synthesis; in fact, if it was added long after the lag phase, growth stimulation occurred without impairment of chlorophyll production. It is suggested that this response of the cells results in an economy in that chlorophyll is not produced until it is a limiting factor.