ENHANCING EFFECT OF ADJUVANTS ON THE ANTITUBERCULOUS IMMUNITY ELICITED IN MICE BY METHANOL EXTRACTS OF TUBERCLE BACILLI

Publisher Website
Google Scholar
Abstract
Cells from young cultures of BCG, killed with 2% phenol and washed with acetone, were used as starting material for studies on vaccination of mice against experimental tuberculosis. An active fraction free of bacillary bodies were separated in a soluble state from the killed cells by prolonged extraction with hot methanol. When this fraction was injected intraperitoneally into mice an increased resistance developed within 2 weeks to infections with bovine and human tubercle bacilli. The immunizing effect of the methanol extract could be enhanced and prolonged by the use of certain unrelated adjuvants, such as (a) a mixture of arlacel and mineral oil, (b) the purified lipopolysaccharide somatic antigen of Salmonella typhosa, (c) commercial pertussis vaccine.