Antibody to sigma 32 cross-reacts with DnaK: association of DnaK protein with Escherichia coli RNA polymerase.
- 1 August 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (15) , 5497-5501
- https://doi.org/10.1073/pnas.85.15.5497
Abstract
A polyclonal antibody to .sigma.32, the heat shock .sigma. factor, has been used to show the presence of low levels of .sigma.32 in Escherichia coli RNA polymerase preparations (E.sigma.70), which explains the observed in vitro activity of E.sigma.70 towards heat shock genes. The .sigma.32 antibody cross-reacts with DnaK, and DnaK has been found associated with purified preparations of both E.sigma.70 and the heat shock RNA polymerase, E.sigma.32.This publication has 27 references indexed in Scilit:
- Correlation between the 32-kDa sigma factor levels and in vitro expression of Escherichia coli heat shock genes.Proceedings of the National Academy of Sciences, 1987
- The heat shock response of E. coli is regulated by changes in the concentration of σ32Nature, 1987
- Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymeraseCell, 1984
- Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factorCell, 1984
- The htpR gene product of E. coli is a sigma factor for heat-shock promotersPublished by Elsevier ,1984
- The dnaK protein modulates the heat-shock response of Escherichia coliCell, 1983
- Positive regulatory gene for temperature-controlled proteins in Escherichia coliBiochemical and Biophysical Research Communications, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving polymin P precipitation and DNA-cellulose chromatographyBiochemistry, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970