The Kinase Insert Domain-containing Receptor (KDR) is Regulated by Shear Stress
- 1 January 2002
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Cardiovascular Journal
- Vol. 36 (6) , 368-372
- https://doi.org/10.1080/140174302762659102
Abstract
Objective : Intimal hyperplasia develops in areas with low shear stress. The aim of the present study was to investigate if the mRNA expression of vascular endothelial growth factor (VEGF), Fms-like tyrosine kinase-1 receptor (Flt-1) and kinase insert domain-containing receptor (KDR) is regulated by shear stress. Design : Endothelial cells from human umbilical veins were in an in vitro system subjected to different levels of shear stress during 1 and 12 h. The mRNA expression of VEGF, Flt-1 and KDR was measured with RT-PCR. eNOS served as positive control and actin as housekeeping gene. Results : The KDR expression was isolated upregulated 3-4 times after 12 h exposure to high shear stress. Conclusion : The genetic expression of KDR is upregulated by shear stress and this response is time dependent.Keywords
This publication has 24 references indexed in Scilit:
- A BAC-Based STS-Content Map Spanning a 35-Mb Region of Human Chromosome 1p35–p36Genomics, 2001
- Distinct Regulation of Vascular Endothelial Growth Factor in Intact Human Conduit Vessels Exposed to Laminar Fluid Shear Stress and PressureBiochemical and Biophysical Research Communications, 2000
- A Novel Function of VEGF Receptor-2 (KDR): Rapid Release of Nitric Oxide in Response to VEGF-A Stimulation in Endothelial CellsBiochemical and Biophysical Research Communications, 1999
- Mechanotransduction in Response to Shear StressJournal of Biological Chemistry, 1999
- VEGF-A Induces Expression of eNOS and iNOS in Endothelial Cells via VEGF Receptor-2 (KDR)Biochemical and Biophysical Research Communications, 1998
- Interaction of genetic deficiency of endothelial nitric oxide, gender, and pregnancy in vascular response to injury in mice.Journal of Clinical Investigation, 1998
- Direct evidence for the importance of endothelium-derived nitric oxide in vascular remodeling.Journal of Clinical Investigation, 1998
- Accelerated Endothelialization by Local Delivery of Recombinant Human Vascular Endothelial Growth Factor Reduces In-Stent Intimal FormationBiochemical and Biophysical Research Communications, 1997
- Shear Stress Increases Heparin-Binding Epidermal Growth Factor-like Growth Factor mRNA Levels in Human Vascular Endothelial CellsBiochemical and Biophysical Research Communications, 1993
- Development and regression of intimal thickening of arterially transplanted autologous vein grafts in dogsJournal of Vascular Surgery, 1987