Molecular Cloning and Sequencing of cDNA Encoding Goat Pre α-Lactalbumin1
- 1 February 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 101 (2) , 511-517
- https://doi.org/10.1093/oxfordjournals.jbchem.a121938
Abstract
In poly(A)+RNA extracted from a lactating goat mammary gland, mRNA of about 750 nucleotides was shown to encode pre α-lactalbumin by using in vitro translation and immunoprecipitation. From the total poly(A)+RNA, the cDNA library was constructed using the Escherichia coli plasmid pUC18; it was screened with the oligodeoxyribonucleotide probe corresponding to the amino acid sequence of Trp60-Gln65 of goat α-lactalbumin. A plasmid containing almost full-length cDNA of goat pre α-lactalbumin, pGLA-1, was identified. The cDNA insert of pGLA-1 comprises 727 base pairs and contains the signal peptide and mature protein sequence.This publication has 11 references indexed in Scilit:
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