Localization of Arginine Vasopressin-Neurophysin II Messenger Ribonucleic Acid in the Hypothalamus of Control and Brattleboro Rats by Hybridization Histochemistry with a Synthetic Pentadecamer Oligonucleotide Probe*
- 1 June 1985
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 116 (6) , 2366-2368
- https://doi.org/10.1210/endo-116-6-2366
Abstract
By using 32P-labeled oligonucleotide probes for in situ DNA-RNA hybridization, the distribution of mRNA for arginine vasopressin-neurophysin II (AVP-NPII) in the rat hypothalamus was defined. With a specific pentadecamer nucleotide probe, strong specific hybridization is seen in supraoptic, suprachiasmatic and paraventricular nuclei in both Sprague-Dawley and Brattleboro rats. This finding of equivalent distribution of AVP-NPII mRNA in Brattleboro and control hypothalami confirms and extends the recent report of equivalent levels of AVP-NPII mRNA by Northern blot analysis.This publication has 2 references indexed in Scilit:
- Structure and comparison of the oxytocin and vasopressin genes from rat.Proceedings of the National Academy of Sciences, 1984
- Structural organization of the rat gene for the arginine vasopressin-neurophysin precursor.The EMBO Journal, 1983