The Oxidation of Malate and Exogenous Reduced Nicotinamide Adenine Dinucleotide by Isolated Plant Mitochondria
- 1 January 1974
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 53 (1) , 104-109
- https://doi.org/10.1104/pp.53.1.104
Abstract
Exogenous NADH oxidation by cauliflower (Brassica oleracea L.) bud mitochondria was sensitive to antimycin A and gave ADP/O ratios of 1.4 to 1.9. In intact mitochondria, NADH-cytochrome c reductase activity was only slightly inhibited by antimycin A. The antimycin-insensitive activity was associated with the outer membrane. Malate oxidation was sensitive to both rotenone and antimycin A and gave ADP/O values of 2.4 to 2.9. However in the presence of added NAD+, malate oxidation displayed similar properties to exogenous NADH oxidation. In both the presence and absence of added NAD+, malate oxidation was dependent on inorganic phosphate and inhibited by 2-n-butyl malonate.Keywords
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