An improved indirect competitive Elisa for aflatoxin m1in milk powders using novel monoclonal antibodies

Publisher Website
Google Scholar
Abstract
Among three newly prepared monoclonal anti‐aflatoxin M1 antibodies, named AM.1, AM.2 and AM.3, both AM.1 and AM.3 possessed high specificity and sensitivity towards aflatoxin M1, while AM.2 exhibited lower specificity. Employing AM.3 and horseradish peroxidase‐labelled second antibody, an improved ELISA was developed with a detection limit of 1.0 pg ml‐1 of aflatoxin M1 in solution. The contents of aflatoxin M1 in powdered milks suspended in water were assayed by the indirect ELISA. The detection limit was 5 pg g‐1dry weight, and no clean‐up procedures were required. The reliability of the present ELISA with monoclonal antibody AM.3 was confirmed with reference powdered milks for aflatoxin M1. A limited ELISA survey showed the presence of aflatoxin M1 in commercial milk powders sampled in France, the US and Thailand at levels of 30–418 pg g‐1, and it was confirmed by an improved HPLC analysis.