HISTOCHEMISTRY OF CONNECTIVE TISSUE: I. THE USE OF ENZYMES AS SPECIFIC HISTOCHEMICAL REAGENTS
Open Access
- 1 September 1953
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 1 (5) , 315-320
- https://doi.org/10.1177/1.5.315
Abstract
This paper presents evidence that the abolition of the staining of a polysaccharide complex in tissue sections can be produced by means other than that of specific hydrolysis, namely, (1) by removal through the action of specific depolymerases, (2) by destruction of proteins to which the stainable material is attached, and (3) by the masking of the stainable groups by the attachment of substances originally present in the enzyme prepn. Consequently one must employ great caution when using and interpreting results based on staining reactions involving enzyme prepns. as histochemical reagents.Keywords
This publication has 9 references indexed in Scilit:
- THE HISTOCHEMISTRY OF CONNECTIVE TISSUE: II. THE EFFECT OF PROTEINS ON THE SELECTIVE STAINING OF MUCOPOLYSACCHARIDES BY BASIC DYESJournal of Histochemistry & Cytochemistry, 1953
- Effect of Cortisone on the Mast Cells of the RatExperimental Biology and Medicine, 1951
- DETERMINATION OF THE CHONDROITINASE ACTIVITY OF BOVINE TESTICULAR PREPARATIONSJournal of Biological Chemistry, 1951
- THE DISTRIBUTION OF ACID MUCOPOLYSACCHARIDES IN MAMMALIAN TISSUES AS REVEALED BY HISTOCHEMICAL METHODSAnnals of the New York Academy of Sciences, 1950
- The organization of ground substance and basement membrane and its significance in tissue injury, disease and growthJournal of Anatomy, 1949
- On the destruction of cytoplasmic basophilia (Ribonucleic acid) and of the metachromatic basophilia of cartilage by the glycogen splitting enzyme malt diastase: A histochemical studyThe Anatomical Record, 1949
- Histochemical studies with malt diastase: Partial separation of the fractions destroying cytoplasmic basophilia (Ribonucleic acid) and the metachromasia of cartilageThe Anatomical Record, 1949
- HISTOCHEMICAL STUDIES ON THE PATHOGENESIS OF FIBRINOID1949
- A MICROCHEMICAL REACTION RESULTING IN THE STAINING OF POLYSACCHARIDE STRUCTURES IN FIXED TISSUE PREPARATIONS1948