Alterations in the Survival of X-irradiated Cells by 2, 4-dinitrophenol Depending on ATP Deprivation

Publisher Website
Google Scholar
Abstract
The dose-survival curve of cultured [mouse] melanoma B16-C2W cells was changed by post-irradiation treatment with 2,4-dinitrophenol [(DNP)a compound which decreases radiation sensitivity]. The parameters of the curves were Do [mean lethal dose] = 147 R and n = 5.6 for untreated cells and Do = 143 R, n = 7.9 and Do = 142 R, n = 2.0 for the cells treated with 10-5 M DNP and 5 .times. 10-5 M DNP in phosphate-buffered saline, respectively. The content of ATP in the cell decreased to 5% of the control level after treatment with either concentration of DNP. The recovery of ATP content was rapid and complete after 2 h incubation in culture medium after the removal of 10-5 M DNP, but was retarded and incomplete after 4 h with 5 .times. 10-5 M DNP. Prolonged ATP deprivation with a high concentration of DNP results in an inhibition of recovery and a reduction in the n-value.