Crystalline Ribulose 1,5-Bisphosphate Carboxylase-Oxygenase from Spinach
- 6 April 1979
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 204 (4388) , 75-77
- https://doi.org/10.1126/science.204.4388.75
Abstract
Spinach fraction I protein (ribulose 1,5-bisphosphate carboxylase-oxy genase, E.C. 4.1.1.39) was crystallized on both an analytical and a preparative scale by vapor diffusion with polyethylene glycol (molecular weight, 6000) used as the precipitant. The identity of the crystalline material with fraction I protein was shown by gel electrophoresis in the presence of sodium dodecyl sulfate and immunological properties. The carboxylase and oxygenase activities copurify during crystallization, and the crystalline enzyme lacks copper and iron.Keywords
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