External monitoring of 203Hg-labelled Dirofilaria immitis microfilariae in mice and dogs
- 1 February 1986
- journal article
- research article
- Published by Cambridge University Press (CUP) in Parasitology
- Vol. 92 (2) , 463-469
- https://doi.org/10.1017/s0031182000064210
Abstract
SUMMARY: Microfilariae of Dirofliaria immitis were labelled with 203Hg 2+in vitro and injected into irradiated mice and Beagle dogs. With irradiated mice it was possible to demonstrate microfilariae present in the blood and to detect 203Hg by external counting as long as 28 days after dosing. The 203Hg 2+ label had a half-time of 4–5 days; the amount of stable mercury in the labelling medium strongly influenced the survival of microfilariae in vivo. In dogs, external counting showed the lungs to be a major location of the microfilariae soon after reinjection into the host. Evidence was obtained that labelled microfilariae can circulate; however, the detection of dispersed microfilariae is difficult because of the relative insensitivity of the detecting system. For radiomercury the accumulation of the inorganic form in the liver and kidneys limits the long-term usefulness of 203Hg 2+ as a label if the organism being studied also accumulates in these organs.This publication has 7 references indexed in Scilit:
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