Ready, Set, Fuse! The Coronavirus Spike Protein and Acquisition of Fusion Competence
Top Cited Papers
Open Access
- 11 April 2012
- Vol. 4 (4) , 557-580
- https://doi.org/10.3390/v4040557
Abstract
Coronavirus-cell entry programs involve virus-cell membrane fusions mediated by viral spike (S) proteins. Coronavirus S proteins acquire membrane fusion competence by receptor interactions, proteolysis, and acidification in endosomes. This review describes our current understanding of the S proteins, their interactions with and their responses to these entry triggers. We focus on receptors and proteases in prompting entry and highlight the type II transmembrane serine proteases (TTSPs) known to activate several virus fusion proteins. These and other proteases are essential cofactors permitting coronavirus infection, conceivably being in proximity to cell-surface receptors and thus poised to split entering spike proteins into the fragments that refold to mediate membrane fusion. The review concludes by noting how understanding of coronavirus entry informs antiviral therapies.Keywords
This publication has 134 references indexed in Scilit:
- Structure and function of the complete internal fusion loop from Ebolavirus glycoprotein 2Proceedings of the National Academy of Sciences, 2011
- Crystal structure of mouse coronavirus receptor-binding domain complexed with its murine receptorProceedings of the National Academy of Sciences, 2011
- Pathogenesis of neurotropic murine coronavirus is multifactorialTrends in Pharmacological Sciences, 2011
- Crystal structure of NL63 respiratory coronavirus receptor-binding domain complexed with its human receptorProceedings of the National Academy of Sciences, 2009
- HIV Enters Cells via Endocytosis and Dynamin-Dependent Fusion with EndosomesCell, 2009
- Activation of the SARS coronavirus spike protein via sequential proteolytic cleavage at two distinct sitesProceedings of the National Academy of Sciences, 2009
- The spike protein of SARS-CoV — a target for vaccine and therapeutic developmentNature Reviews Microbiology, 2009
- Synthetic recombinant bat SARS-like coronavirus is infectious in cultured cells and in miceProceedings of the National Academy of Sciences, 2008
- Importance of SARS-CoV spike protein Trp-rich region in viral infectivityBiochemical and Biophysical Research Communications, 2008
- Structural basis of viral invasion: lessons from paramyxovirus FCurrent Opinion in Structural Biology, 2007