Evidence of 15 S–S Bridges in the Hinge Region of Human 530

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Abstract
By amino acid analyses and radioactive titration of cleaved S–S bonds on reduction, it was shown that the hinge region of IgG3 and its F(ab′)2 fragment seem to contain 15 S–S bridges. About three of the bridges are not reduced by 0.01 M dithiothreitol in aqueous buffer, and, in addition, this treatment cleaved the F(ab′)2 fragment into two Fab′ subunits. The observation, therefore, may support the idea that the γ3‐chain contains an extra intrachain disulphide loop.