ANTAGONISM BY DIBUTYRYL ADENOSINE CYCLIC 3',5'-MONOPHOSPHATE AND TESTOSTERONE OF CELL ROUNDING REACTIONS
Open Access
- 1 November 1973
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 59 (2) , 471-479
- https://doi.org/10.1083/jcb.59.2.471
Abstract
In an attempt to understand further the mechanism of the morphological and functional "reverse transformation" of CHO-K1 cells induced by dibutyryl adenosine cyclic 3',5'-monophosphate (cAMP) and testosterone, the kinetics of variation in the susceptibility of cells to rounding after the addition or deletion of dibutyryl cAMP and testosterone have been investigated. Changes in susceptibility to cell rounding upon removal of divalent cations or pulse exposure to concanavalin A were complete within 0.5-1 h after addition or deletion of drug. In comparison, the gross conversion of CHO-K1 cells from epithelial- to fibroblast-like morphology after drug treatment or the converse change after drug removal required 8 or 4 h, respectively. The effects on cell rounding are not caused by an effect of dibutyryl cAMP upon cell growth rate. Inhibitor experiments indicate that the changes investigated do not require continued RNA or protein synthesis and are not prevented by agents which depolymerize microtubules.Keywords
This publication has 25 references indexed in Scilit:
- EVIDENCE FOR PLEIOTROPIC CHANGES IN LINES OF CHINESE HAMSTER OVARY CELLS RESISTANT TO CONCANAVALIN A AND PHYTOHEMAGGLUTININ-PThe Journal of cell biology, 1973
- Surface Changes in Temperature-Sensitive Simian Virus 40-Transformed CellsProceedings of the National Academy of Sciences, 1973
- The effect of inhibitors of RNA and protein synthesis on dibutyryl cyclic AMP mediated morphological transformations of Chinese hamster ovary cells in vitroBiochemical and Biophysical Research Communications, 1973
- Further Changes in Differentiation State Accompanying the Conversion of Chinese Hamster Cells to Fibroblastic Form by Dibutyryl Adenosine Cyclic 3′:5′-Monophosphate and HormonesProceedings of the National Academy of Sciences, 1971
- Restoration of Contact-Inhibited Growth to Transformed Cells by Dibutyryl Adenosine 3′:5′-Cyclic MonophosphateProceedings of the National Academy of Sciences, 1971
- Unusual Fragments in the Subunit Structure of Concanavalin AProceedings of the National Academy of Sciences, 1971
- Morphological Transformation of Chinese Hamster Cells by Dibutyryl Adenosine Cyclic 3′:5′-Monophosphate and TestosteroneProceedings of the National Academy of Sciences, 1971
- Cyclic Membrane Changes in Animal Cells: Transformed Cells Permanently Display a Surface Architecture Detected in Normal Cells Only during MitosisProceedings of the National Academy of Sciences, 1971
- Restoration of Normal Growth by Covering of Agglutinin Sites on Tumour Cell SurfaceNature, 1970
- Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.Proceedings of the National Academy of Sciences, 1968