Some Effects of Ultraviolet Irradiation on Yeast Cells (Candida utilis)

Abstract
Effects of UV irradiation on yeast cells (Candidautilis) have been studied by UV photomicrography and by biochemical analysis. Morphological changes consist of enlargement of the vacuole, followed by a decline in cell size, and, eventually, coagulation of cellular protein. The vacuolar membrane loses its selective permeability in the same fashion as the cell membrane; vacuolar constituents are released into the cytoplasm and thence into the surrounding medium along with cytoplasmic constituents. The effect of UV irradiation is not immediate; after a sufficient dose, some time is required for the release of cellular constituents into the surrounding medium. There appears to be no selective specificity in the types of compounds released, because at 25[degree]C essentially all material absorbing at 260 m/i is lost by the cells if the exposure to UV irradiation has been high enough.