Rescue of noncultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus.

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Abstract
Fastidious human rotaviruses that did not undergo productive infection in tissue culture [African green monkey kidney cells] were rescued by genetic reassortment during mixed infection with a temperature-sensitive (ts) mutant of a cultivatable bovine rotavirus. The genes of the fastidious rotavirus that restricted growth in vitro were replaced by the corresponding genes from a tissue culture-adapted rotavirus. Genetically reassorted viruses that grew to high titer were recovered and were neutralized specifically by hyperimmune guinea pig 1 or type 2 human rotavirus antiserum. Preliminary RNA analysis of these clones disclosed that they were viruses with reassorted genes. [Rotavirus is the single most important cause of infectious infantile gastroenteritis in developed countries.].