Subpopulations of rat and mouse thoracic duct small lymphocytes in the Salmonella flagellar antigen system.

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  • 1 December 1969
    • journal article
    • Vol. 17  (6) , 955-67
Abstract
The purpose of the present experiments was to determine whether populations of lymphocytes from mouse or rat thoracic duct lymph deprived of the larger, dividing cells (large and medium lymphocytes) could still transfer adequate primary or secondary adoptive immune responses using syngeneic irradiated hosts. The transfer system used involved the antigen polymerized flagellin from Salmonella adelaide and assay of anti-H antibody levels in the recipients. Two methods of preparation of `small lymphocytes' were used, namely the glass bead column filtration method of Shortman and the agitated culture method of Gowans and Uhr (1966). Both of these yield lymphocyte fractions essentially free of dividing cells.