Measurement of Trazodone Using Solid-Phase Extraction and Wide-Bore Capillary Gas Chromatography with Nitrogen-Selective Detection

Abstract
A method for the determination of the antidepressant drug trazodone is presented. 8-Hydroxyloxapine is used as the Internal standard. A simple solid-phase extraction procedure utilizing disposable reversed-phase C19 columns is described. Samples are analyzed by go chromatography with nitrogen-selective detection using a wide-bore capillary column with a permanently bonded, nonpolar stationary phase. The assay possesses linearity to 3.0 µg/mL, sensitivity to at least 0.25 µg/mL, recovery averaging 96%, and between-run precision reflected by a CV of 5.6%. We conclude that the method reported here is ideally suited for monitoring therapeutic and toxic levels of trazodone.