Effect of increased glycogen synthase kinase-3 activity upon the maturation of the amyloid precursor protein in transfected cells

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Abstract
ABERRANT control of protein phosphorylation is an important feature in Alzheimer's disease pathology. The action of glycogen synthase kinase-3β (GSK-3β) on the maturation and phosphorylation of an amyloid precursor protein-reporter construct (APP-REP) was studied in transfected COS-7 cells. Elevation of GSK-3β activity by enzyme over-expression resulted in an increase in the level of mature forms of co-expressed APP-REP. This effect was not associated with an increased level of APP-REP phosphorylation at Thr743, an in vitro GSK-3β phosphorylation site. These findings suggest that GSK-3 β activity may indirectly increase cellular maturation of APP, which may subsequently result in altered production of β-amyloid protein.