Polymorphism of HLA-DR beta chains in DR4, -7, and -9 haplotypes: implications for the mechanisms of allelic variation.
- 1 December 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (23) , 9149-9153
- https://doi.org/10.1073/pnas.83.23.9149
Abstract
We have isolated and sequenced cDNA clones corresponding to the DR beta 1 and DR beta 2 loci from two homozygous B-cell lines typed as DR7 (Burkhart) and DR9 (ISK). These nucleotide sequences were compared to beta 1 and beta 2 chains of other DR haplotypes. The first-domain sequences of beta 2 chains are identical in DR4 and DR7 haplotypes. In addition, there is strong sequence homology within the 3' untranslated regions of beta 1 genes from DR4, -7, and -9 haplotypes, thus confirming the close evolutionary relationship among these three haplotypes. In contrast, the first-domain sequences of beta 1 molecules from these haplotypes are very different from each other and do not reflect the DR4, -7, -9 family relationship. Two explanations for the differences in degree of diversity between beta 1 and beta 2 chains are suggested. The differences may be a consequence of selection pressures; this implies functional differences for products of the beta 1 and beta 2 loci. Alternatively, closely linked segments of the human class II region may differ in their underlying rates of variation, independent of selection pressures, and this may in part account for the extraordinary diversity found in the beta 1 first domain.This publication has 22 references indexed in Scilit:
- Identification of common molecular subsequencesPublished by Elsevier ,2004
- Polymorphism of human Ia antigens: gene conversion between two DR β loci results in a new HLA-D/DR specificityNature, 1986
- Gene organization and recombinational hotspots in the murine major histocompatibility complexCell, 1986
- Structural organization of the DR subregion of the human major histocompatibility complex.Proceedings of the National Academy of Sciences, 1985
- Molecular characterization of MT3 antigens by two-dimensional gel electrophoresis, NH2-terminal amino acid sequence analysis, and southern blot analysis.Proceedings of the National Academy of Sciences, 1985
- Structure, Sequence and Polymorphism in the HLA‐D RegionImmunological Reviews, 1985
- DNA and Protein Studies of HLA Class II Molecules: Their Relationship to T Cell RecognitionImmunological Reviews, 1985
- Nucleotide sequence of an HLA-DQ alpha chain derived from a DRw9 cell line: genetic and evolutionary implications.Proceedings of the National Academy of Sciences, 1985
- A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322Gene, 1981
- A general method applicable to the search for similarities in the amino acid sequence of two proteinsJournal of Molecular Biology, 1970