Hydrochloric acid as a Fixative for root Tip Chromosomes
- 1 January 1949
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 24 (2) , 95-97
- https://doi.org/10.3109/10520294909139587
Abstract
After pretreatment with 0.2 to 0.3% colchicine at 26° C. for 2 hours root tips are fixed and macerated in a 1/10 dilution of concentrated hydrochloric acid at 60° C. for 10 to 14 minutes, washed, transferred to a staining dish with aceto-orcein or another aceto-stain for about 10 minutes, immersed in a drop of the stain, covered and squashed. The preparations may subsqeuently be made permanent.Keywords
This publication has 4 references indexed in Scilit:
- Improvements in plant cytological technique. IIThe Botanical Review, 1947
- Prefixing with Paradichlorobenzene to Facilitate Chromosome StudyStain Technology, 1945
- Cytological Methods for Crepis SpeciesStain Technology, 1942
- OBSERVATIONS ON THE IMMEDIATE EFFECTS OF COLCHICINEJournal of Heredity, 1939