Mechanism of Aminonucleoside-Induced Nephrosis in the Rat. II. Metabolism of Aminonucleoside-8-C14.

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Abstract
Utilizing chromatographic and carbon-14 labeling techniques, detailed studies were conducted on the chemical nature of carbon-14 activity found in acid-soluble nucleotide, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA) fractions isolated from both kidney and liver tissue of rats treated with the aminonucleoside, 6-dimethylamino-9-(3[image]-amino-3[image]-doxy-[beta]-D-ribofuranosyl)-purine-8-Cl4 No significant differences were noted between tissues obtained from rats treated for periods of 10 days with small daily doses of the aminonucleoside and those from rats sacrificed as early as 3 hours after stimulation with a single large dose of the labeled compound. Only the adenosine and guanosine phosphates of the acid-soluble nucleotide fractions, and the adenine and guanine bases of RNA and DNA hydrolysates were labeled with carbon-14. No evidence was obtained for the conversion of the intact aminonucleoside to the corresponding nucleotide, or for the incorporation of the 6-dimethylamino- or the 6-methylamino-purine moieties into ribonucleic acid or deoxyribonucleic acid of kidney or liver tissue.