Mechanism of Aminonucleoside-Induced Nephrosis in the Rat. I. Metabolism of Tritiated Aminonucleoside.

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Abstract
1. The preparation and metabolism of radiochemically pure aminonucleoside, 6-dimethylamino-9-(3′-amino-3′-deoxy-β-D-ribofuranosyl)-purine, labeled with tritium by the Wilzbach procedure, is described and discussed. Data are presented for the distribution of radioactivity in body fluids and urine as well as in kidney and liver nucleic acids. 2. Study of the chemical nature of tritium activity in an acid-soluble nucleotide fraction, prepared from pooled kidney tissue of rats rendered nephrotic with the tritiated aminonucleoside, indicates that the rat is capable of metabolizing the latter into labeled products utilizable in the biosynthesis of adenosine and cytidine nucleotides. 3. Labeling of these nucleotides, which cannot be accounted for in terms of an exchange mechanism involving the hydrogens of body water, is considered in terms of alternative metabolic pathways.