Recovery of protein from tear fluid stored in cellulose sponges
- 1 January 1987
- journal article
- Published by Taylor & Francis in Current Eye Research
- Vol. 6 (4) , 585-588
- https://doi.org/10.3109/02713688709025217
Abstract
Human tear fluid collected in glass capillaries was absorbed in cellulose sponges. After various time intervals the recovery was measured for total protein, amylase (AMY), lysozyme (LZM), immunoglobulin A (IgA) and lactoferrin (LF). After one day storage at 4 degrees C of moistened sponges of at least 92% recovery was observed. Storage for longer periods at 4 degrees C up to seven days showed a decreasing recovery for total protein, AMY and IgA. Tear collection with sponges and storage of wet sponges for maximally one day at 4 degrees C followed by centrifugation or storage of the fluid at -20 degrees C are reliable ways of sample treatment.Keywords
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