Ribosomal ribonucleic acid isolated from Salmonella typhimurium: absence of the intact 23S species

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Abstract
RNA isolated by 4 distinct methods and from a variety of S. typhimurium strains lacked intact 23S rRNA. On sucrose gradients which minimize aggregation, the vast majority of S. typhimurium rRNA sedimented as a 16S peak with a 14S shoulder. RNA from this region of the gradient was resolved into 3 discrete bands by electrophoresis in formamide. Two very minor S. typhimurium RNA peaks were resolved at 21S and 10S on sucrode gradients, and each peak formed discrete bands in electrophoresis. If S. typhimurium does possess an intact 23S rRNA species; this species is extremely labile. The absence of isolatable S. typhimurium 23S rRNA possibly reflected in vivo processing of the rRNA before isolation. Under certain conditions, S. typhimurium rRNA formed discrete aggregates which sedimented similarly to intact Escherichia coli 23S rRNA.

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