Ploidy distribution in experimental liver carcinogenesis in mice
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 9 (1) , 59-63
- https://doi.org/10.1093/carcin/9.1.59
Abstract
There have been several reports on the distribution of DNA in premalignant and malignant liver cells after carcinogen treatment in rodents. The most striking result so far is that ploidy distributions seems to depend more on experimental protocols and less on carcinogenesis per se . The present study demonstrates that an evaluation by means of flow cytometry and stereological image analysis yields results showing similar ploidy distributions throughout the known sequence of tissue changes seen during liver cancer development. Furthermore, our results demonstrate inter-individual differences between the lesions in each of the steps in these tissue changes, (foci, nodules and hepatocarcinomas). The present study gives no reason to conclude that changes in ploidy distribution are essential in liver carcinogenesis.Keywords
This publication has 13 references indexed in Scilit:
- Karyometric analysis of liver cell dysplasia and hepatocellular carcinoma. Evidence against precancerous nature of liver cell dysplasiaCancer, 1985
- Irreversible depression in the ratio of tetraploid:diploid liver nuclei in rats treated with 3'-methyl-4-diraethylaminoazobenzene (3'M)Carcinogenesis: Integrative Cancer Research, 1985
- Emergence of a population of small, diploid hepatocytes during hepatocarcinogenesisCarcinogenesis: Integrative Cancer Research, 1984
- Ploidy patterns in hepatic tumors induced by mirexExperimental and Molecular Pathology, 1983
- A Detergent‐trypsin method for the preparation of nuclei for flow cytometric DNA analysisCytometry, 1983
- DNA Content of Liver Cell Nuclei of N-2-Fluorenylacetamide-Induced Altered Foci and Neoplasms in Rats and Human Hyperplastic Foci2JNCI Journal of the National Cancer Institute, 1982
- Studies on DNA content of hepatocytes in cirrhosis and hepatoma by means of microspectrophotometry and radioautographyHistochemistry and Cell Biology, 1982
- CYTOPHOTOMETRIC AND CYTOGENETIC CHARACTERISTICS OF PRIMARY HEPATOMAS INDUCED IN WISTAR RATS BY AFLATOXIN-B11981
- Flow Cytometry: A High-Resolution Instrument for EveryoneScience, 1979
- Quantitative Kinetics of Development of N-2-Fluorenylacetamide-Induced, Altered (Hyperplastic) Hepatocellular Foci Resistant to Iron Accumulation and of Their Reversion or Persistence Following Removal of Carcinogen23JNCI Journal of the National Cancer Institute, 1978