Role of 3-Nitropropanoic Acid in Nitrate Formation by Aspergillus flavus

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Abstract
Doxtader , K. G. (Cornell University, Ithaca, N.Y.), and M. Alexander . Role of 3-nitropropanoic acid in nitrate formation by Aspergillus flavus . J. Bacteriol. 91: 1186–1191. 1966.— Aspergillus flavus formed nitrate, 3-nitropropanoic acid (3-NPA), kojic acid, and a substance tentatively identified as N -formyl- N -hydroxy-glycine during growth in a medium with ammonium as sole nitrogen source. The concentration of the nitro compound reached a maximum prior to the appearance of nitrate; the 3-NPA level subsequently decreased with a concomitant increase in nitrate concentration. Replacement cultures of A. flavus produced nitrate from culture filtrates containing 3-NPA or from synthetic 3-NPA but not when supplied with fresh ammonium-sucrose medium, the nitrate-nitrogen formed being equivalent to 50% of the quantity of the 3-NPA-nitrogen initially present. Neither nitrate nor 3-NPA was synthesized by the fungus during growth in media with low p H or low ammonium concentrations. It is proposed that 3-NPA is either an intermediate or is in equilibrium with an intermediate in nitrification by the fungus.