IDENTIFICATION OF TARGET PROTEINS PARTICIPATING IN A LECTIN-DEPENDENT MACROPHAGE-MEDIATED CYTO-TOXIC REACTION

Google Scholar
  • 1 January 1985
    • journal article
    • research article
    • Vol. 45  (1) , 288-292
Abstract
Membrane proteins of mouse macrophages and mammary tumor cells having affinity to Sarcophaga peregrina lectin were isolated by affinity chromatography. The electrophoretic profiles and antigenicities of lectin-binding proteins from macrophages and tumor cells were different. Antibody raised against tumor cell lectin-binding proteins inhibited both the binding of the lectin to tumor cells and the lectin-dependent macrophage-mediated cytotoxic reaction. It did not inhibit the binding of the lectin to macrophages. The same lectin molecule evidently transmitted different stimuli to macrophages and tumor cells via different receptor proteins.