Liposome‐Mediated DNA uptake by sperm cells
- 1 November 1991
- journal article
- transgenics
- Published by Wiley in Molecular Reproduction and Development
- Vol. 30 (3) , 194-200
- https://doi.org/10.1002/mrd.1080300305
Abstract
To investigate the potential use of sperm cells as vectors to transfer exogenous DNA via the fertilization of oocytes into the germ line of mice, we have used liposomes to transfect DNA into the sperm head. Although the DNA transfer into sperm mediated by liposomes was very efficient and no obvious reduction in the fertilization frequency of oocytes could be detected, we were unable to generate transgenic mice by this method.Keywords
This publication has 6 references indexed in Scilit:
- Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery.The EMBO Journal, 1991
- No simple solution for making transgenic miceCell, 1989
- Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA.Proceedings of the National Academy of Sciences, 1989
- Sperm cells as vectors for introducing foreign DNA into eggs: Genetic transformation of miceCell, 1989
- Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.Proceedings of the National Academy of Sciences, 1987
- GERM-LINE TRANSFORMATION OF MICEAnnual Review of Genetics, 1986