Abstract
The following insect cell lines were cultured continuously in serum-free media; [Papilio xuthus] NIAS-PX-58, [Mamestra brassicae] NIAS-MB-32, [M. brassicae] NIAS-MaBr-85, [Aedes albopictus] NIAS-AeAl-2, [Leucania separata] NIAS-LeSe-11 and [Sarcophaga peregrina (Takahashi, 1980)] NIH-SaPe-4. These cells also grow in media lacking cholesterol. The cells cultured in serum-free medium were susceptible to low and ultralow temperature storage.