Ruthenium(II)−Phenanthroline−Biotin Complexes: Synthesis and Luminescence Enhancement upon Binding to Avidin
- 24 August 2004
- journal article
- Published by American Chemical Society (ACS) in Bioconjugate Chemistry
- Vol. 15 (5) , 949-953
- https://doi.org/10.1021/bc049919o
Abstract
We report the synthesis, characterization, and avidin-binding properties of two novel ruthenium complexes, [Ru(bpy)2(phen-biotin)][PF6]21 and [Ru(phen)2(phen-biotin)][PF6]22 (bpy = 2,2‘-bipyridine; phen = 1,10-phenanthroline, phen-biotin = 5-(10-amidobiotinyl)-1,10-phenanthroline)). We demonstrate that both biotinylated compounds bind to avidin through their biotin moieties with high affinity and in a 4:1 ratio. The binding of compounds 1 and 2 to avidin results in an enhancement in luminescence intensity (∼1.4×, ∼1.6×, respectively), relative to the unbound biotinylated ruthenium complexes. This behavior is markedly different from biotinylated organic dyes, whose fluorescence is quenched upon binding to avidin. Thus, ruthenium−biotin complexes 1 and 2 can form the basis of new, simplified biotin−avidin assays, which involve luminescence detection of the relevant biotinylated molecule through cross-linking with avidin.Keywords
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