PRODUCTION OF A LYMPHOCYTE-PROLIFERATION POTENTIATING FACTOR BY PURIFIED POLYMORPHONUCLEAR LEUKOCYTES FROM MICE AND RABBITS

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  • 1 January 1984
    • journal article
    • research article
    • Vol. 53  (4) , 683-692
Abstract
Highly pure polymorphonuclear leukocytes (PMN) were prepared from peritoneal exudate cells which were induced by an i.p. injection of casein into C3H mice and rabbits. The PMN were tested for the production of a lymphocyte proliferation potentiating factor with various stimulations in vitro. In both animal species, the purified PMN from the inflammatory site 3 h after injection (3-h PMN) produced the factor upon stimulation with kaolin; the purified PMN from the lesion 24 h after injection (24-h PMN) did not. The 3-h PMN produced the potentiating factor during a relatively earlier period after in vitro stimulation with kaolin. Protein synthesis inhibitors did not inhibit the factor production, suggesting the release of a preformed factor from 3-h PMN. The effect of kaolin did not appear to be simply due to its cytotoxicity, because the release as not parallel with 51Cr-release from the PMN. The factor produced by mouse PMN had an MW of 15,000-25,000; it consisted of 2 isoelectrophoretically distinct factors, i.e, isoelectric point (pI) 9.4 and 5.4. The rabbit PMN factor was slightly smaller (MW 10,000-20,000) and was composed of 3 pI species, i.e., 7.2, 5.4 and 4.5.

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