Activity of Mitochondrial Preparations Obtained from Faris Sweet Lemon Fruit

Abstract
Citric acid occurred in a lower concentration than malic acid in the Faris sweet lemon. Oxidative phosphorylation was obtained with citrate, a-ketoglutarate, succinate, and malate as substrates. When citrate-1, 5-C14 was used as a substrate, labeled a-ketoglutarate was found after 60 minutes and labeled malate after 120 minutes. When succinate-1,4-C14 was used as a substrate, malate and fumarate were fojnd after 60 minutes and also citrate after 120 minutes. When pyruvate-3-C14 was used as a substrate together with a sparker acid, citrate, [alpha]-ketoglutarate, and malate were found after 120 minutes. These findings, together with the inhibitory effects of cyanide and malonate, indicated the citric acid cycle was operative in this variety of lemon.