Biochemical studies of taste sensation: monoclonal antibody against L-alanine binding activity of catfish taste epithelium.
- 1 December 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (23) , 7595-7597
- https://doi.org/10.1073/pnas.79.23.7595
Abstract
The L-alanine taste receptors of the channel catfish Ictalurus punctatus provide a useful biochemical model for studying taste receptor mechanisms. Mouse hybridomas that synthesize monoclonal antibodies have been produced. The antigen used to activate mouse spleen cells was the plasma membrane fraction obtained from the taste receptor-containing epithelium of the channel catfish. The spleen cells were fused with myeloma cells, Sp2/0-Ag14, to form hybridomas. To demonstrate inhibition of ligand binding by the product of these hybridomas, a catfish membrane fraction (fraction P2) was incubated with the antibody-containing preparation prior to assaying for L-[3H]alanine binding activity. We thereby demonstrated inhibition of binding of the taste ligand L-alanine to fraction P2. This approach should prove useful in further studies of receptor binding and transduction events in taste receptors.This publication has 16 references indexed in Scilit:
- Monoclonal Antibodies: A Powerful New Tool in Biology and MedicineAnnual Review of Biochemistry, 1981
- Biochemical studies of taste sensation. VII. Enhancement of taste stimulus binding to a catfish taste receptor preparation by prior exposure to the stimulusJournal of Neurobiology, 1979
- Biochemical studies of taste sensation: binding to taste tissue of 3H-labeled monellin, a sweet-tasting protein.Proceedings of the National Academy of Sciences, 1979
- Biochemical studies of taste sensation—VIII. Partial characterization of alanine-binding taste receptor sites of catfish Ictalurus punctatus using mercurials, sulfhydryl reagents, trypsin and phospholipase CComparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1979
- A Quantitative Disc Radioimmunoassay for Antibodies Directed Against Membrane-Associated AntigensPublished by Springer Nature ,1979
- Hybrid Plasmacytoma Production: Fusions with Adult Spleen Cells, Monoclonal Spleen Fragments, Neonatal Spleen Cells and Human Spleen CellsPublished by Springer Nature ,1979
- A better cell line for making hybridomas secreting specific antibodiesNature, 1978
- Derivation of specific antibody‐producing tissue culture and tumor lines by cell fusionEuropean Journal of Immunology, 1976
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964