A COMPARATIVE-STUDY OF LYSOSOMAL ENZYME-ACTIVITY IN MONOCYTES AND KUPFFER CELLS ISOLATED SIMULTANEOUSLY IN A RAT MODEL OF LIVER-INJURY

  • 1 January 1981
    • journal article
    • research article
    • Vol. 43  (2) , 376-380
Abstract
Macrophages were isolated and cultured in vitro from normal rat livers and from livers into which macrophages were recruited in vivo, following an i.v. injection of killed Corynebacterium parvum [Propionibacterium acnes]. Simultaneously, peripheral blood monocytes were isolated and cultured in vitro. After 24 h in culture, supernatants and cell lysates were harvested and the activity of a lysosomal enzyme, N-acetyl-glucosaminidase (NAG), measured. NAG activity in the recruited tissue macrophages cell lysates was significantly higher than that measured in control tissue macrophages. Increased NAG activity was observed in the supernatants from the recruited macrophages. The NAG activity in cell lysates and supernatants of peripheral monocytes was not significantly changed after C. parvum injection. In this animal model, measurement of a lysosomal enzyme produced by peripheral monocytes did not reflect the magnitude of the changes observed for the tissue macrophages.