Isolation and Identification of Novel Tocotrienols from Rice Bran with Hypocholesterolemic, Antioxidant, and Antitumor Properties

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Abstract
Two novel tocotrienols were isolated from stabilized and heated rice bran, apart from the known α-, β-, γ-, and δ-tocopherols and tocotrienols. These new tocotrienols were separated by HPLC, using a normal phase silica column. Their structures were determined by ultraviolet, infrared, nuclear magnetic resonance, circular dichroism, and high-resolution mass spectroscopies and established as desmethyl tocotrienol [3,4-dihydro-2-methyl-2-(4,8,12-trimethyltrideca-3‘(E),7‘(E),11‘-trienyl)-2H-1-benzopyran-6-ol] and didesmethy tocotrienol [3,4-dihydro-2-(4,8,12-trimethyltrideca-3‘(E),7‘(E),11‘-trienyl)-2H-1-benzopyran-6-ol]. These tocotrienols significantly lowered serum total and LDL cholesterol levels and inhibited HMG-CoA reductase activity in chickens. They had much greater in vitro antioxidant activities and greater suppression of B16 melanoma cell proliferation than α-tocopherol and known tocotrienols. Results indicated that the number and position of methyl substituents in tocotrienols affect their hypocholesterolemic, antioxidant, and antitumor properties. Keywords: Stabilized and heated rice bran; novel tocotrienols; hypocholesterolemic; antioxidant and antitumor activities
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