Malignant transformation of NIH-3T3 and CV-1 cells by a helical mycoplasma,Spiroplasma mirum, strain SMCA
- 1 December 1986
- journal article
- rapid communications-in-cell-biology
- Published by Springer Nature in In Vitro Cellular & Developmental Biology
- Vol. 22 (12) , 756-762
- https://doi.org/10.1007/bf02621093
Abstract
Summary A helical mycoplasma,Spiroplasma mirum strain SMCA, produced malignant transformation in mouse NIH 3T3 cells and monkey kidney CV-1 cells. The transformed cells exhibited morphological changes consistent with the transformed phenotype, grew in soft agar and produced tumors in athymic and BALB/c mice. Transmission electron microscopy revealed structures morphologically similar to mycoplasmas present in the cytoplasm of transformed but not untransformed 3T cells. The time of inoculation ofS. mirum SMCA to 3T3 cells and the passage level of 3T3 cells affected transformation.Keywords
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