Appearance of Soluble and Gross-Reactive Complement-Fixing Antigens on Treatment of Poliovirus with Formalin.

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Abstract
Poliovirus in tissue culture fluid was treated with dilute formadehyde and fractionated by ultracen-trifugation. Most of the complement-fixing antigen in the active virus preparations could be sedimented at 105,000 x gravity for 2 hours. However, in the formalin-treated samples a soluble antigen appeared which remained in the supernatant fluid. The active virus preparations used were almost completely type specific in their complement-fixing reactions, but after treatment with formaldehyde, cross reactions were found between all types.