Abstract
A medium was developed for the simultaneous culture of loblolly pine callus and C. fusiforme of basidiospore origin. Gresshoff and Doy medium 1 was modified by using different N concentrations, growth regulators, 2% glucose, adding 0.05% peptone, and 0.05% of an 80% ethanol-soluble fraction of yeast extract. This medium permitted good growth of both cultures without serious detriment to either organism during interactive studies. Light and temperature conditions suitable for survival and growth of both organisms are given.

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