BINDING OF ANTI-DNA ANTIBODIES AS MEASURED FLUOROMETRICALLY BY ETHIDIUM-BROMIDE
- 1 January 1978
- journal article
- research article
- Vol. 5 (4) , 391-398
Abstract
The phenanthridine dye ethidium bromide (EB) intercalates with double-stranded (ds) DNA resulting in an enhancement of fluorescence. Single-stranded (ss) DNA does not show this fluorescent enhancement. Purified Ig[immunoglobulin]G from patients with systemic lupus erythematosus (SLE) containing anti-dsDNA antibodies [Ab] competes with EB for binding to DNA resulting in a decrease in fluorescence. Ab which bind ssDNA in the Millipore filter radioimmunoassay displace EB from dsDNA showing that antigenic determinants are available for binding in the ds molecule. This study introduces the EB assay and presents a comparison with the Millipore filter assay.This publication has 11 references indexed in Scilit:
- Avidity of antibodies in sleArthritis & Rheumatism, 1976
- Immunology of DNA. II. The effect of size and structure of the antigen on the farr assayJournal of Immunological Methods, 1976
- Immunology of DNA. I. The influence of reaction conditions on the farr assay as used for the detection of anti-ds DNAJournal of Immunological Methods, 1976
- DETERMINATION OF ANTI-DNA ANTIBODIES BY A MODIFIED I-125-LABELED DNA-BINDING TEST - ELIMINATION OF NONSPECIFIC BINDING OF DNA TO NON-IMMUNOGLOBULIN BASIC-PROTEINS BY USING AN ANIONIC DETERGENT1976
- Profiles of Antinuclear Antibodies in Systemic Rheumatic DiseasesAnnals of Internal Medicine, 1975
- Procainamide-induced antibodies to nucleoprotein, denatured and native DNA in human subjectsClinical Immunology and Immunopathology, 1975
- Antibodies to Native and Denatured Deoxyribonucleic Acid in Systemic Lupus Erythematosus*Journal of Clinical Investigation, 1967
- Deoxybonucleic acid (DNA) and antibodies to DNA in the serum of patients with systemic lupus erythematosus.Journal of Clinical Investigation, 1966
- Complex formation between ethidium bromide and nucleic acidsJournal of Molecular Biology, 1965
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951