Apolipoprotein D and Platelet-Derived Growth Factor-BB Synergism Mediates Vascular Smooth Muscle Cell Migration
- 23 July 2004
- journal article
- research article
- Published by Wolters Kluwer Health in Circulation Research
- Vol. 95 (2) , 179-186
- https://doi.org/10.1161/01.res.0000135482.74178.14
Abstract
We identified apolipoprotein (apo)D in a search for proteins upregulated in a posttranscriptional manner similar to fibronectin in motile smooth muscle cells (SMCs). To address the function of apoD in SMCs, we cloned a partial apoD cDNA from ovine aortic (Ao) SMCs using RT-PCR. We documented a 2.5-fold increase in apoD protein but no increase in apoD mRNA in Ao SMCs 48 hours after a multiwound migration assay ( P P P P <0.05). This was unrelated to increased phosphorylation of ERK1/2 or of phospholipase C-γ1, but correlated with enhanced Rac1 activation. This study shows that apoD can be expressed or taken up by SMCs and can regulate their motility in response to growth factors.Keywords
This publication has 43 references indexed in Scilit:
- Apolipoprotein D Inhibits Platelet-Derived Growth Factor-BB–Induced Vascular Smooth Muscle Cell Proliferated by Preventing Translocation of Phosphorylated Extracellular Signal Regulated Kinase 1/2 to the NucleusArteriosclerosis, Thrombosis, and Vascular Biology, 2003
- Leptin promotes invasiveness of kidney and colonic epithelial cells via phosphoinositide 3‐kinase‐, Rho‐, and Rac‐dependent signaling pathwaysThe FASEB Journal, 2000
- Overactivation of Phospholipase C-γ1 Renders Platelet-derived Growth Factor β-Receptor-expressing Cells Independent of the Phosphatidylinositol 3-Kinase Pathway for ChemotaxisPublished by Elsevier ,1999
- Microtubule-associated protein 1 light chain 3 is a fibronectin mRNA-binding protein linked to mRNA translation in lamb vascular smooth muscle cells.Journal of Clinical Investigation, 1997
- Involvement of Phosphatidylinositide 3′-Kinase and Rac in Platelet-Derived Growth Factor-Induced Actin Reorganization and ChemotaxisExperimental Cell Research, 1997
- Arachidonic acid binds to apolipoprotein D: implications for the protein's functionFEBS Letters, 1995
- The lipocalin protein family: A role in cell regulationFEBS Letters, 1994
- Fibronectin, hyaluronan, and a hyaluronan binding protein contribute to increased ductus arteriosus smooth muscle cell migrationDevelopmental Biology, 1991
- Qualitative and quantitative differences in protein synthesis comparing fetal lamb ductus arteriosus endothelium and smooth muscle with cells from adjacent vascular sitesDevelopmental Biology, 1988
- Isolation and partial characterization of apolipoprotein D: A new protein moiety of the human plasma lipoprotein systemFEBS Letters, 1973