PURIFICATION AND PARTIAL CHARACTERIZATION OF AN ENDO-β1,3-GLUCANASE FROM GREEN MALT
Open Access
- 4 March 1976
- journal article
- research article
- Published by Chartered Institute of Brewers and Distillers in Journal of the Institute of Brewing
- Vol. 82 (2) , 64-67
- https://doi.org/10.1002/j.2050-0416.1976.tb03726.x
Abstract
An endo-β1,3-glucanase from a green malt extract was purified by DEAE- and CM-cellulose ion exchange chromatography followed by molecular sieve chromatography on BioGel P-100. A final enzyme preparation had two protein components on disc electrophoresis, one of which was in-active. The enzyme had a pH optimum of 5·0 for activity on laminarin and 5·8 on carboxymethyl pachyman. The activity was stable up to 60°C and was stimulated by NaCl. The isoelectric point of the enzyme was 9·8.Keywords
This publication has 11 references indexed in Scilit:
- THE ACTION OF ENDO-β-1,3-GLUCANASES ON BARLEY AND MALT β-GLUCANSJournal of the Institute of Brewing, 1974
- DEVELOPMENT OF CYTOLYTIC ENZYME ACTIVITIES DURING GERMINATION OF DIFFERENT BARLEYSCanadian Journal of Plant Science, 1974
- Quantitative densitometry of 1–50 μg protein in acrylamide gel slabs with coomassie blueAnalytical Biochemistry, 1972
- A LOW pH DISC ELECTROPHORESIS SYSTEM FOR STUDYING α-AMYLASE ENZYMESJournal of the Institute of Brewing, 1971
- Preparation and Purification of Glucanase and Chitinase from Bean LeavesPlant Physiology, 1971
- I. DETERMINATION OF BARLEY AND MALT NITROGEN CONTENT USING AN AUTOANALYSER TECHNIQUEJournal of the Institute of Brewing, 1970
- THE CHROMATOGRAPHIC PROPERTIES OF BARLEY AND MALT β-AMYLASESJournal of the Institute of Brewing, 1969
- The mechanism of action of malt β-glucanasesArchives of Biochemistry and Biophysics, 1964
- β-1,3-Glucan hydrolases from the grape vine (Vitis vinifera) and other plantsPhytochemistry, 1962
- Variable Gradient Device for ChromatographyAnalytical Chemistry, 1959